Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking.
Identifieur interne : 002D87 ( Main/Exploration ); précédent : 002D86; suivant : 002D88Distinct forelimb and hind limb stepping impairments in unilateral dopamine-depleted rats: use of the rotorod as a method for the qualitative analysis of skilled walking.
Auteurs : Ian Q. Whishaw [Canada] ; Katie Li ; Paul A. Whishaw ; Bogdan Gorny ; Gerlinde A. MetzSource :
- Journal of neuroscience methods [ 0165-0270 ] ; 2003.
English descriptors
- KwdEn :
- Animals, Dopamine (deficiency), Exercise Test (instrumentation), Exercise Test (methods), Female, Forelimb (physiopathology), Hindlimb (physiopathology), Lameness, Animal (chemically induced), Lameness, Animal (etiology), Lameness, Animal (physiopathology), Motor Skills, Movement, Oxidopamine, Parkinsonian Disorders (chemically induced), Parkinsonian Disorders (complications), Parkinsonian Disorders (physiopathology), Posture, Rats, Rats, Long-Evans, Reference Values, Task Performance and Analysis, Video Recording (methods), Walking.
- MESH :
- chemical , deficiency : Dopamine.
- chemically induced : Lameness, Animal, Parkinsonian Disorders.
- complications : Parkinsonian Disorders.
- etiology : Lameness, Animal.
- instrumentation : Exercise Test.
- methods : Exercise Test, Video Recording.
- physiopathology : Forelimb, Hindlimb, Lameness, Animal, Parkinsonian Disorders.
- Animals, Female, Motor Skills, Movement, Oxidopamine, Posture, Rats, Rats, Long-Evans, Reference Values, Task Performance and Analysis, Walking.
Abstract
The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.
PubMed: 12788498
Affiliations:
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Whishaw</name><affiliation wicri:level="1"><nlm:affiliation>Department of Psychology and Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge, 4401 University Dr., Alberta, Lethbridge, Canada T1K 3M4. whishaw@uleth.ca</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Psychology and Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge, 4401 University Dr., Alberta, Lethbridge</wicri:regionArea><wicri:noRegion>Lethbridge</wicri:noRegion></affiliation></author><author><name sortKey="Li, Katie" sort="Li, Katie" uniqKey="Li K" first="Katie" last="Li">Katie Li</name></author><author><name sortKey="Whishaw, Paul A" sort="Whishaw, Paul A" uniqKey="Whishaw P" first="Paul A" last="Whishaw">Paul A. Whishaw</name></author><author><name sortKey="Gorny, Bogdan" sort="Gorny, Bogdan" uniqKey="Gorny B" first="Bogdan" last="Gorny">Bogdan Gorny</name></author><author><name sortKey="Metz, Gerlinde A" sort="Metz, Gerlinde A" uniqKey="Metz G" first="Gerlinde A" last="Metz">Gerlinde A. 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Whishaw</name><affiliation wicri:level="1"><nlm:affiliation>Department of Psychology and Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge, 4401 University Dr., Alberta, Lethbridge, Canada T1K 3M4. whishaw@uleth.ca</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Psychology and Neuroscience, Canadian Centre for Behavioural Neuroscience, University of Lethbridge, 4401 University Dr., Alberta, Lethbridge</wicri:regionArea><wicri:noRegion>Lethbridge</wicri:noRegion></affiliation></author><author><name sortKey="Li, Katie" sort="Li, Katie" uniqKey="Li K" first="Katie" last="Li">Katie Li</name></author><author><name sortKey="Whishaw, Paul A" sort="Whishaw, Paul A" uniqKey="Whishaw P" first="Paul A" last="Whishaw">Paul A. Whishaw</name></author><author><name sortKey="Gorny, Bogdan" sort="Gorny, Bogdan" uniqKey="Gorny B" first="Bogdan" last="Gorny">Bogdan Gorny</name></author><author><name sortKey="Metz, Gerlinde A" sort="Metz, Gerlinde A" uniqKey="Metz G" first="Gerlinde A" last="Metz">Gerlinde A. Metz</name></author></analytic><series><title level="j">Journal of neuroscience methods</title><idno type="ISSN">0165-0270</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Dopamine (deficiency)</term><term>Exercise Test (instrumentation)</term><term>Exercise Test (methods)</term><term>Female</term><term>Forelimb (physiopathology)</term><term>Hindlimb (physiopathology)</term><term>Lameness, Animal (chemically induced)</term><term>Lameness, Animal (etiology)</term><term>Lameness, Animal (physiopathology)</term><term>Motor Skills</term><term>Movement</term><term>Oxidopamine</term><term>Parkinsonian Disorders (chemically induced)</term><term>Parkinsonian Disorders (complications)</term><term>Parkinsonian Disorders (physiopathology)</term><term>Posture</term><term>Rats</term><term>Rats, Long-Evans</term><term>Reference Values</term><term>Task Performance and Analysis</term><term>Video Recording (methods)</term><term>Walking</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Dopamine</term></keywords><keywords scheme="MESH" qualifier="chemically induced" xml:lang="en"><term>Lameness, Animal</term><term>Parkinsonian Disorders</term></keywords><keywords scheme="MESH" qualifier="complications" xml:lang="en"><term>Parkinsonian Disorders</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Lameness, Animal</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Exercise Test</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Exercise Test</term><term>Video Recording</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Forelimb</term><term>Hindlimb</term><term>Lameness, Animal</term><term>Parkinsonian Disorders</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Female</term><term>Motor Skills</term><term>Movement</term><term>Oxidopamine</term><term>Posture</term><term>Rats</term><term>Rats, Long-Evans</term><term>Reference Values</term><term>Task Performance and Analysis</term><term>Walking</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The rotorod test, in which animals walk on a rotating drum, is widely used to assess motor status in laboratory rodents. Performance is measured by the duration that an animal stays up on the drum as a function of drum speed. Here we report that the task provides a rich source of information about qualitative aspects of walking movements. Because movements are performed in a fixed location, they can readily be examined using high-speed video recording methods. The present study was undertaken to examine the potential of the rotorod to reveal qualitative changes in the walking movements of hemi-Parkinson analogue rats, produced by injection of 6-hydroxydopamine (6-OHDA) into the nigrostriatal bundle to deplete nigrostriatal dopamine (DA). Beginning on the day following surgery and then periodically over the next two months, the rats were filmed from frontal, lateral, and posterior views as they walked on the rotorod. Behavior was analyzed by frame-by-frame replay of the video records. Rating scales of stepping behavior indicated that the hemi-Parkinson rats were chronically impaired in their posture and in the use of the limbs contralateral to the DA-depletion. The contralateral limbs not only displayed postural and movement abnormalities, they participated less in initiating and sustaining propulsion than did the ipsilateral limbs. These findings not only reveal new deficits secondary to unilateral DA-depletion, but also show that the rotorod can provide a robust tool for the qualitative analysis of movement.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Gorny, Bogdan" sort="Gorny, Bogdan" uniqKey="Gorny B" first="Bogdan" last="Gorny">Bogdan Gorny</name><name sortKey="Li, Katie" sort="Li, Katie" uniqKey="Li K" first="Katie" last="Li">Katie Li</name><name sortKey="Metz, Gerlinde A" sort="Metz, Gerlinde A" uniqKey="Metz G" first="Gerlinde A" last="Metz">Gerlinde A. Metz</name><name sortKey="Whishaw, Paul A" sort="Whishaw, Paul A" uniqKey="Whishaw P" first="Paul A" last="Whishaw">Paul A. Whishaw</name></noCountry><country name="Canada"><noRegion><name sortKey="Whishaw, Ian Q" sort="Whishaw, Ian Q" uniqKey="Whishaw I" first="Ian Q" last="Whishaw">Ian Q. Whishaw</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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